Title
Hydrolysis studies of flaxseed extract by High Performance Liquid Chromatography
Authors
D. DRAGANESCU G. DODI I. STOICA and M. I. POPA

Received In memory of Acad. Cristofor I. Simionescu, a man of outstanding qualities as a teacher, researcher and school founder
Published Volume 51 Issue 9-10 September-December
Keywords flaxseed extract, SDG, acidic hydrolysis, enzymatic hydrolysis, HPLC

Abstract
Flaxseed is the richest dietary source of enterolignan precursors, primarily secoisolariciresinol diglucoside (SDG), with potential health benefits and an excellent nutrient profile. In this study, SDG oligomers in a flaxseed extract were hydrolysed to break first ester linkages for the release of SDG and then glycosidic bonds for the release of secoisolariciresinol (SECO). The hydrolysates of SDG oligomers are complex, therefore, different pathways were investigated and evaluated by high-performance liquid chromatography (HPLC) in order to improve the separation and determination of flaxseed components: acidic and enzymatic hydrolysis using ten different enzymes, such as β glucuronidase, sulphatase, laccase, lignin peroxidases, Bakezyme® Concreate, Panamore® Golden, Veron®292, Veron®393, Gluzyme®10000 BG and Fungamyl®2500 SG. The obtained results revealed that the chemical hydrolysis, as well as the enzymatic treatment using laccase, determined the liberation of SDG oligomers from flaxseed lignan macromolecules in different quantities. The application of all the other enzymes did not change significantly the composition of the flaxseed extract.