Title
Comparative study on the polyphenols and pectin of three Eryngium species and their antimicrobial activity
Authors
SIMONA CONEA LAURIAN VLASE and IOAN CHIRILA

Published Volume 50 Issue 3-4 March-April
Keywords Eryngium, polyphenols, phenolic acids, flavonoids, antimicrobial

Abstract
The polyphenols and pectin from the aerial parts of Eryngium planum, E. campestre and E. maritimum were investigated. For screening the polyphenols, the HPLC-UV-MS method was employed, while pectin was quantified by the gravimetric method. Pectin isolation was performed using hot water extraction or by a selective extraction with 4 different solvents. Isoquercitrin, quercitrin, kaempferol, quercetol, chlorogenic acid, caffeic acid, p-coumaric acid and ferulic acid were the polyphenols detected in all Eryngium species. The pectin content yielded by hot water extraction varied substantially among the species, being the highest in E. maritimum (8.8%), medium in E. planum (4.65%) and the lowest in E. campestre (1.7%). The selective extraction method revealed the highest pectin content in E. campestre (26.74%), followed by E. maritimum (17.8%) and E. planum (12.21%). The screening of antimicrobial activity confirmed a moderate effect on Staphyloccocus aureus and S. epidermidis, as well as a high bacteriostatic effect on Pseudomonas aeruginosa. The effect of the E. campestre tincture was the most pronounced.