Title
Effect of laccase dosage on enzymatic hydrolysis of steam-exploded wheat straw
Authors
ALFREDO OLIVA-TARAVILLA ELIA TOMAS-PEJO MARIE DEMUEZ CRISTINA GONZALEZ-FERNANDEZ and MERCEDES BALLESTEROS

Published Volume 50 Issue 3-4 March-April
Keywords lignocellulose, wheat straw, enzymatic hydrolysis, laccase detoxification, ATR-FTIR

Abstract
When laccases are applied for detoxifying steam-exploded slurries, enzymatic hydrolysis is affected. In this study, 10% (w/v) of dry weight (DW) slurry or water insoluble solid fraction were subjected to 48 h enzymatic hydrolysis in the presence of different laccase loadings (0.1, 0.5, 5 or 10 IU/g DW). All laccase dosages higher than 0.1 IU/g DW significantly inhibited glucose recovery. Nevertheless, half of the amount of phenolic compounds present in steam exploded slurries was removed with 0.1 IU/g DW of laccase, while 75% of phenolic compounds were efficiently eliminated when employing 0.5 IU/g DW laccase. These findings suggested that, in order to avoid enzymatic hydrolysis inhibition, laccase dosage has to be kept as low as possible for detoxifying steam-exploded wheat straw slurry.