Title
Enzymatic hydrolysis of waste office paper for ethanol production by Spathaspora passalidarum
Authors
J. M. T. S. ROCHA B. R. A. ALENCAR H. G. MOTA and E. R. GOUVEIA

Received August 14, 2014
Published Volume 50 Issue 2 February
Keywords fermentation, cellulose, lignocellulosic, bioethanol, waste paper

Abstract
Waste office paper was used as a feedstock for the production of bioethanol. Saccharification ofwaste office paper by sequential acid and enzyme hydrolysis produced a high quality hydrolysate that ensured its fermentability to produce ethanol. The optimal saccharification process resulted in total 42 g/L of glucose (63% cellulose conversion). Spathaspora passalidarum HMD 14.2 was able to coferment with glucose (98%), xylose (74%) and cellobiose (45%) released in the hydrolysate from waste office paper. There was an ethanol production of13 g/L. An ethanol yield of0.42 g/g, fermentation efficiency of 82% and volumetric ethanol productivity of 0.57 g/L/h were achieved. In our study, ethanol production was about 300% higher than that using acid hydrolysis and Spathaspora passalidarum HMD 14.2.