Title
HPLC method for the evaluation of chromatographic conditions for separation of new xanthine derivatives
Authors
ADRIANA TAMBA BOGDAN CIOROIU LENUTA PROFIRE and MIHAI IOAN LAZAR

Received November 4, 2012
Published Volume 48 Issue 1-2 January-February
Keywords HPLC, methylxanthines

Abstract
The aim of this study is to develop a suitable HPLC method to determine with efficiency and selectivity the individual components of a series of 8 xanthine derivative compounds. HPLC analysis was performed using a high performance liquid chromatography system and a Phenomenex C18 column (octadecylsilyl) with 250 mm length, 4.6 mm internal diameter, 5 µm particle size and high pH stability.
The UV spectra for the eight new xanthine derivatives were drawn. The retention times, number of theoretical plates and purity factor in different solvents were also established by using the HPLC method. The method is suitable for determining the xanthine derivatives from different pharmaceutical coated matrices, based on their physicochemical properties, such as the affinity to polar compounds or vegetable proteins.