Title
Determination of glucose content in cellulose derived from banana stem (Musa spp.) using UV-Vis spectrophotometry and HPLC methods
Authors
MUHAIMIN and BAYU WIYANTOKO

Received September 5, 2025
Published Volume 59 Issue 9-10 October-December
Keywords banana stem, glucose, microcrystalline cellulose, UV-Vis spectrophotometry, HPLC

Abstract
This study investigates the glucose content in hydrolyzed microcrystalline cellulose (MCC) derived from banana stem (Musa spp.) in a comparative analysis by two techniques: UV-Vis spectrophotometry and high-performance liquid chromatography (HPLC). MCC was isolated by delignification, bleaching, hydrothermal re-alkalization, and HCl hydrolysis, then characterized using physicochemical tests, FTIR, and XRD. Glucose levels were analyzed using two spectrophotometric methods (phenol-sulfuric acid and sulfuric acid–UV) and two HPLC systems (UV detector and refractive index detector). Results showed that banana stem MCC had properties similar to Avicel PH 101, but contained higher moisture and ash. The sulfuric acid–UV method provided accurate glucose quantification and is suitable for routine analysis, while HPLC-UV offered the highest sensitivity for detecting low glucose concentrations. Thus, UV-Vis spectrophotometry is more practical, whereas HPLC-UV is ideal for highly sensitive analyses.