Title
Ultrasound extraction and characterization of bioactive compounds obtained from different organs of Datura innoxia
Authors
GEORGIANA MARDARE (BALUSESCU), LILIANA LAZAR and TEODOR MALUTAN

Received December 19, 2024
Published Volume 59 Issue 1-2 January-February
Keywords biomass, biorefinery, green extraction, chromatography, spectrophotometry, spectroscopy, secondary metabolites

Abstract
In this work, a green technique for bioactive compounds extraction from different vegetative organs (leaves, flowers, seeds, stem and root) of Datura innoxia, assisted by ultrasound (UAE), was performed using ethanol and 1-butanol as solvents. The chemical compositions of the extracts and quantitative determination of alkaloids (scopolamine) were investigated using different methods: Fourier transform infrared (FTIR) spectroscopy, fluorescence spectrophotometry (FL), ultraviolet-visible (UV-VIS) spectroscopy and gas chromatography with flame ionization detection (GC-FID). Based on the effect of solvent polarity on extraction, the results indicated that 1-butanol extracts show high extraction efficiency of lipophilic compounds in a non-polar solvent. Moreover, the results show a high abundance of the main classes of secondary metabolites (terpenes, alkaloids, phenolics), and alkanes, esters, ketones, alcohols, carbohydrates and polysaccharides (lignin and cellulose) were among the classes of compounds identified in matrix extracts. Taking into account the mentioned aspects, this study demonstrated that UAE can be adopted to obtain secondary metabolites with active principles of pharmacological and cosmetic importance from Datura innoxia dry biomass for sustainable biorefining.